Figure 3. Data averaging reduces noise and
enhances contrast between structures. A: A rolling 3-frame
average within a 120 h post fertilization (hpf) embryo improves
visualization of soft tissue structures in the gut while blurring the
sharp features of the eye and ear. B: A side by side comparison
of a single frame and a 120 frame averaged within the brain of a 24 hpf
embryo demonstrates that aggressive averaging of repeated line scans
increased visualization of soft tissue features and reduced noise.