Figure 3. Data averaging reduces noise and enhances contrast between structures. A: A rolling 3-frame average within a 120 h post fertilization (hpf) embryo improves visualization of soft tissue structures in the gut while blurring the sharp features of the eye and ear. B: A side by side comparison of a single frame and a 120 frame averaged within the brain of a 24 hpf embryo demonstrates that aggressive averaging of repeated line scans increased visualization of soft tissue features and reduced noise.