Figure 2. Rotation of a 3D image dataset of a 120 hpf embryo. SD-OCT can acquire a 3 dimensional quantitative description of the tissues within zebrafish embryos. These data allow the non-invasive visualization of the entire animal, as well as cross-sectional slices in any orientation through the animal at any stage of development, all without sacrificing the animal or noticeably impeding development. This gross anatomical visualization of the zebrafish was rendered in 3D-View, using the maximum intensity projection display.