Figure 5. Immunofluorescence staining of semithin sections from explants cocultured with the mononuclear fraction. Antibody against glial fibrillary acidic protein (GFAP; red) and DAPI dye (blue) were used. At 3 (A), 6 (B), and 9 (C) days of culture, the Müller cells were wider and more positive for GFAP than controls. After 9 days in culture (C), numerous Müller cell GFAP+ processes invaded the subretinal space, distributed in several fibrous layers (arrows). Scale bar: 20 µm. Abbreviations: GCL is ganglion cell layer; ILM is inner limiting membrane; INL is inner nuclear layer; NFL: nerve fiber layer; ONL is outer nuclear layer.