Table 2 of Li, Mol Vis 2008; 14:2136-2142. Table 2 of Li, Mol Vis 2008; 14:2136-2142.
Table 2. Details of the primers and enzymes used for PCR-RFLP genotyping.
SNPs |
dbSNP ID |
Forward primer |
Reverse primer |
Tm (°C) |
Restriction enzyme |
|---|---|---|---|---|---|
| −169C/T(fcrl3_3) | rs7528684 | GGAAAATAATACA AATGTACAGATTA | GGCTTTAAAA AACGGTGGTAC | 56.9 | BsmFI |
| −110A/G(fcrl3_4) | rs11264799 | CTCAATCCCGGT AGTGATACA | CTCATAACAAC TTATGTGAGA | 56.9 | Ple I |
| +358C/G(fcrl3_5) | rs945635 | TTATAGCCCATCTA CTCACTCAGGATCA | CCGGGATTGAGA TACAAACAGCATTT | 60.3 | HaeIII |
| +1381A/G(fcrl3_6) | rs3761959 | TCCGACTTTTTCA GTCTCTAGGTTTT | TGATAGCAGCACTA GCTTGGACATTCA | 60.3 | MspI |