Figure 3 of Mimura, Mol Vis 2008; 14:197-203.

Figure 3. Formation of secondary sphere. (A, B) Secondary spheres were generated after the dissociation of primary spheres derived from peripheral or central keratocytes. Scale bar=100 μm. (C) The re-plating efficiency from primary to secondary colonies was higher for spheres derived from the peripheral stroma than for those from the central stroma (p=0.000025, unpaired t-test).