Figure 5. In situ hybridization of IMPG2 transcripts. In situ hybridization studies were performed on retinal sections from 2-month-old wild-type mice with antisense (A) and control sense (B) probes. Retinal sections of wild-type (C,D) and Rpe65^−/− (E,F) mice at 2 (C,E) and 6 months of age (D,F) were hybridized with the antisense probe to detect IMPG2 transcripts. Abbreviations: retinal pigment epithelium (rpe); photoreceptor outer segments (pos); outer nuclear layer (onl): outer plexiform layer (opl); inner nuclear layer (inl); inner plexiform layer (ipl); retinal ganglion cell layer. Scale bars equal 30 μm (A,B) and 40 μm (C-F).