Figure 4. In situ hybridization of NGC transcripts. In situ hybridization studies were performed on retinal sections of 2-month-old wild-type mice with antisense (A) and control sense (B) probes. Retinal sections of wild-type (C,D) and Rpe65^−/− (E,F) mice at 2 (C,E) and 6 months of age (D,F) were hybridized with the antisense probe to detect NGC transcripts. NGC mRNA is expressed in the RPE cell layer (G; arrows). Abbreviations: retinal pigment epithelium (rpe): photoreceptor outer segments (pos); outer nuclear layer (onl); outer plexiform layer (opl); inner nuclear layer (inl); inner plexiform layer (ipl); retinal ganglion cell layer (gcl). Scale bars represents 30 μm (A,B) and 40 μm (C-F).