Table 1 of Ranjan, Mol Vis 2008; 14:2056-2066.

Table 1. The IC50 values of various antigens as determined by non-competitive ELISA using affinity purified IgG fraction from the antisera raised against human total lens proteins.

Antigens
(Ag)		 IC50 (based on
regression analysis)		 r2
value
Human total lens protein 55 ng 0.99
Human HMW+α 16.45 ng 0.99
Human HMW+α-gly 273 ng 0.95
Human β 37.82 ng 0.99
Human β-gly 260 ng 0.91
Rat γ 105.34 ng 0.98
Rat γ-gly 313 ng 0.97

Both glycated and non-glycated lens crystallins were used for the analysis. The affinity purified IgG fraction was used at a dilution of 1:40, 000. The regression coefficients (r2) indicate the linearity of the displacement curve.

Ranjan, Mol Vis 2008; 14:2056-2066. http://www.molvis.org/molvis/v14/a242

©2008 Molecular Vision http://www.molvis.org/molvis/

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