Table 2 of Natoli, Mol Vis 2008; 14:1983-1994.

Table 2. Measures of sample and data quality.

Experiment #1 Day 0 Day 3 Day 7 Day 14
GAPDH 5′/3′ ratios 1.03 0.87 0.81 2.16
Background 43.41 55.04 37.47 39.53
Noise (rawQ) 1.34 1.69 1.16 1.19
Scale factor 1.765 1.524 2.785 2.586
Percent present 53.4 55 47.8 48
Experiment #2 Day 0 Day 3 Day 7 Day 14
GAPDH 5′/3′ ratios 1.11 0.79 1.03 2.44
Background 43.77 53.3 42.76 40.21
Noise (rawQ) 1.36 1.67 1.31 1.24
Scale factor 1.68 1.533 1.808 2.376
Perecent present 54.7 54.9 51.4 47.5

The quality of each chip was evaluated using standardized criteria including 5′/3′ ratio of GAPDH, background, noise (rawQ), scale factor, and percent present. All values met the required criteria.

Natoli, Mol Vis 2008; 14:1983-1994. http://www.molvis.org/molvis/v14/a234

©2008 Molecular Vision http://www.molvis.org/molvis/

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