Table 2 of Fang, Mol Vis 2008; 14:1974-1982.

Table 2. Primers for amplifying and sequencing GPR143 genomic segments.



Exon

Forward primer (5′- 3′)

Reverse primer (5′- 3′)		 Product
size (bp)		 Annealing
temperature
(°C)
1A CTCCTCCGCCCGCCCAAGCATCAC CCCAGGCAGCCGAGAAGGTC 464 68
1B CCGCGCCTAGGGACCTTCTGCT AACCCGCGGGCCTCTCGTCCTCAC 399 70
2 CTTTCTTCCTTTTCCCTCCTTGTC GTTTGCTGCTGCTGCGATTTG 360 58
3 CACGTGCGGCTTCCTGAC TTGGCCTCTTATAAAAATGA 385 56
4 GGGCTTTCCTCTGTGTACATTTTC CCCTGAGACAACGGCCTAACC 334 60
5 GCATTTCCCTTTTTGTTCTCATCC AGGCCTGCACATTTTCATTTATTG 406 58
6 TTGCTTCCTGCCCCTCTGG ACTTGCTCCCCTGTCCTCTGT 400 60
7 TGCACCTGGCCCTCTTAGTTTC TCAGGAGGCCAAGACAGAGGAT 441 60
8A AAACCAACCCACCAACCAGTCAAC GCATGCTCAGGGCTTCGTCA 395 60
8B CCAGCCCAGGGATTTCTCTT ACCCCGCCATGCACAGGAC 329 60
9 AGCTGATGACAAACCTGCTAG CCCTTTCTCCTATCCTAAAG 330 58
FAM5 CCTAGGGACCGGATGGGACAAC CTCGCCAACAGTTACACAGCTC 487 60
FAM6 GTCCCCTTTCTTCCCTTCTCTACT GTGGTGTTTGTCTTTGGTTTTGTG 297 60
SSCP-1 AGCCACGCAGCTCGTGCTGAGCTTCCAGCC CCCAGGCGCTGATCAGATTCCAACCCGCGG 250 70
SSCP-2 CATCCTCTTATCTTGACTTCC CCCAGAGAGCTTCCCTAGT 224 58
SSCP-3 CATGTTCTCTTTACCTGCTGC AGTGAGACCTTGTCTCTGAAG 227 56

Listed are primer sequences, sizes of PCR products, and annealing temperature used for the amplification. Primers 1-9 were used to amplify and sequence the GPR143 genimic segments. Primers FAM5 and FAM6 were used to detect the deletion boundaries in families 5 and 6, respectively. Primers SSCP-1 to 3 were used to amplify exons 1, 5, and 7 for heterduplex-SSCP analysis.

Fang, Mol Vis 2008; 14:1974-1982. http://www.molvis.org/molvis/v14/a233

©2008 Molecular Vision http://www.molvis.org/molvis/

ISSN 1090-0535