Figure 2. Angiographic analysis of the effect of pSilencer^siVEGF on the murine model of retinal neovascularization. Retinal flatmounts were examined by fluorescein dextran angiography. A: Shown is a retinal flatmount of mice raised in room air. B: In this retinal flatmount of mice exposed to hyperoxia, neovascular tufts appear as hyperfluorescence at the junction between the perfused and the nonperfused area. Arrow points to retinal neovascularization, and star marks an area of nonperfusion. C: In this retinal flatmount of hyperoxia exposed mice injected with pSilencer null vector, retinal neovascularization and nonperfusion area were not attenuated. D: Shown is a retinal flatmount of hyperoxia-exposed mice injected with pSilencer^siVEGF. The amount of neovascular tufts was markedly reduced and the area of nonperfusion was diminished.