Figure 3. Downregulation of LIMK1
decreases actin polymerization and focal adhesion formation. Corneal
fibroblasts transfected with TransIT-TKO transfection reagent, a 100 nM
scrambled or LIMK1 siRNA for 72 h were fixed and permeablized.
The cells were stained for actin (green color, using Alexa 488
phalloidin) and paxillin (red color, using anti-paxillin and Cy3-goat
anti-mouse IgG) where indicated. The nuclei were stained with DAPI
(blue color). Note the reduction of actin stress fibers and
paxillin-positive focal adhesions in LIMK1 siRNA-transfected
cells. Bar, 20 μm.