Figure 2. LIMK1-targeted siRNA blocks corneal fibroblast migration. A-D: Phase contrast micrographs demonstrating migration of (A) untreated cells; (B) cells treated with 100 nM scrambled control siRNA; (C) cells treated with 50 nM LIMK1-targeted siRNA; (D) cells treated with 100 nM LIMK1-tareted siRNA into the scratched wound area (marked by dotted white lines). E: Bar graph showing mean percentage of scratch-wounded area covered by migrating corneal fibroblasts in each specimen (n=10); error bars represent standard error of the mean. Asterisks denote values significantly different from those of samples treated with scrambled control siRNA (p<0.0001 [n=10] compared to scrambled siRNA). Experiments were repeated 3 times, yielding similar results.