Figure 3. Zymographic analysis of samples after the enrichment of the minor endonuclease. A: The zymogram shows progressively increasing endonuclease activity: lane 1, pooled G-100 fractions “A”; lane 2, fractions with activity pooled from ConA-Sepharose; lane 3, fractions with activity pooled after oligo (dT) cellulose chromatography. B: Zymographic gel stained with silver. Lanes 1–3 are the same as A. The arrow indicates the position of the minor tear endonuclease activity. M, molecular weight markers as shown. C: Zymogram of the minor tear endonuclease (~34 kDa) in the presence (lane 1) and absence (lane 2) of β−mercaptoethanol.