Figure 1. Quantitation of DNA in human tears. The oligogreen dye assay provides a measurement of the amount of DNA in freshly collected tears. The fluorescence enhancement of the dye bound to DNA is measured, λex=480 nm and λem=520 nm. The results for tear samples (■, diluted 2.125X) are plotted on a standard curve of oligonucleotides (●) of known concentration. The numbers in parentheses are the concentrations of the oligonucleotides in tears corrected for dilution.