Figure 7 of Zhang, Mol Vis 2008; 14:1929-1939.


Figure 7. Expression of K8 and MUC5AC. Immunofluorescence staining with K8 (A-C,E,H) and MUC5AC (D,F,I) was performed with ocular surface whole mounts of GFP-Dstncorn1 mice (A,C,E-I) and CAG-EGFP mice (B,D) at the indicated ages. For the GFP-Dstncorn1 specimens, only the cornea is shown, and for the CAG-EGFP specimens, a superior half of the ocular surface containing cornea and conjunctiva up to the lid margin is shown together with an approximate position of the cornea-limbus boundary line shown as dashed lines. B and D show that K8 and MUC5AC are completely absent in normal CAG-EGFP corneas even at an advanced age while they are present in wide areas of the conjunctiva. A, C, and E show that K8 is widely distributed in GFP-Dstncorn1 corneas at all ages. E and F show the same GFP-Dstncorn1 cornea (73 weeks old) with double staining with K8 and MUC5AC, respectively. G, H, and I show high power images of the same 73-week-old cornea with triple staining with DAPI, K8, and MUC5AC. Most of the MUC5AC positive cells are also positive with K8. Bars: 500 µm (A-F), 20 µm (G-I).