Figure 5. Distribution of corneal LRCs. LRCs were determined in a 
20-week-old CAG-EGFP cornea (A,B) and a 28-week-old GFP-Dstncorn1 cornea 
(C,D) by BrdU pulse chase labeling followed by BrdU immunohistochemistry. LRCs were determined by BrdU pulse chase labeling followed by BrdU immunohistochemistry. They were plotted manually under the microscope, surveying the entire inferior half of the cornea. Cuts were made to mount specimens flat either from the corneal side (A) or from the limbal side (C). An approximate position of a border between the cornea and the limbus is indicated by arrows and dashed lines. High power images of representative BrdU positive cells are also shown for a CAG-EGFP cornea (B) and a GFP-Dstn^corn1 cornea (D). A shows a representation of three CAG-EGFP corneas, and C shows a representative of eight GFP-Dstn^corn1 corneas that all showed similar results. Bar: 500 µm (A,C); 20 µm (B,D).