Figure 3. Overproduction of ETS-1 and ETS-2 mRNAs and proteins in a mouse model of ocular neoplasm (Tyrp-1), with respect to control mice (WT) between P15 and three months. mRNA levels were determined by semi-quantitative RT–PCR. The values shown correspond to the levels of overproduction of ETS-1 and ETS-2 with respect to levels in normal control mice at the same age. The graphs (A and C) show the intensity level of each PCR band, corresponding to ETS-1 (A) and ETS-2 (C) as measured by densitometric analysis. The cyclophilin is used as an internal standard control. The PCR bands for ETS-1 (B) and ETS-2 (D) are shown. The relative levels are calculated as the ratio of intensities of each ETS band to the cyclophilin band for each lane. Error bars indicate SEM. Proteins were detected by western blotting (E and F). Levels were quantified using Scion image and normalized using β-actin as an internal standard (G and H).