Figure 2 of De la Houssaye, Mol Vis 2008; 14:1912-1928.


Figure 2. Distribution of ETS transcription factor proteins in mouse adult retina, cornea, and ciliary body as determined by immunohistofluorescence. Immunohistofluorescence for ETS-1 and ETS-2 is shown (A-D). ETS-1 (A and C) is present in the ganglion cell layer (GCL) and inner nuclear layer (INL) with intense homogeneous staining in the outer nuclear layer (ONL). It is also detected in the photoreceptor inner segment (PIS) and retinal pigment epithelium (RPE). ETS-2 (B and D) is also present in significant amounts in the GCL. Weak ETS-2 immunoreactivity is observed in the INL and ONL. In these layers, at higher magnification, very few cell bodies are immunostained. The immunolabeling detected seems to be restricted to the nuclear membranes whereas the nucleoplasm seems to be devoid of ETS-2 immunolabeling. In contrast to what was observed for the retinal distribution of ETS-1 immunoreactivity, strong ETS-2 immunolabeling is observed in the inner plexiform layer (IPL) and outer plexiform layer (OPL). As observed for ETS-1, the PIS and RPE display marked ETS-2 immunostaining, although the RPE seems to be less strongly stained for ETS-2 than for ETS-1. ETS-1 (E) and ETS-2 (F) protein distributions are shown in the adult retina with the DAB immunostaining protocol. We observed ETS-1 (G) and ETS-2 (H) immunolabeling in the adult ciliary body. In the ciliary body, ETS-1 and ETS-2 proteins are detected in the nonpigmented ciliary epithelial cells (NPCE). No significant labeling is observed in the pigmented ciliary epithelial cells (PCE). ETS-1 immunolocalisation was detected in the adult cornea at low (I) and high (K) magnification. ETS-2 immunolocalisation was detected in the adult cornea at low (J) and high (L) magnification. In the cornea, ETS-1 and ETS-2 immunoreactivities are observed in the epithelium (CEp), the stroma (CS), the stromal keratocytes (ke, arrows), and the endothelium (CEn).