Figure 1. Distribution of ETS-1 and ETS-2 mRNAs and proteins in the adult mouse eye. The ETS-1 and ETS-2 mRNAs were detected in adult mouse tissue sections by in situ hybridization (HIS), and their distributions are shown (A and E, respectively). The ETS-1 and ETS-2 proteins were detected by immunohistofluorescence (IHC) in adult mouse tissue sections. The specific immunostaining patterns obtained are also shown (B and F, respectively). The retinal tissue distributions of ETS-1 (B) and ETS-2 (F) proteins appeared to be somewhat different whereas the distribution of the transcripts corresponding to these proteins (A and E) seemed to be similar in adult mouse retina. The ETS-1 (C)and ETS-2 (G) mRNAs show the negative control in adult mouse tissue sections by in situ hybridization obtained with sense riboprobes. The ETS-1 (D) and ETS-2 (H) immunohistofluorescence shows the negative control in adult mouse tissue section. The results were obtained with the second antibody alone. The nucleus was counterstained with DAPI. The scale bar represents 100 µm. GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer, RPE: retinal pigment epithelium; PIS: photoreceptor inner segment.