Table 2 of Srivastava, Mol Vis 2008; 14:1872-1885.

Table 2. Purification of α-crystalllin-associated proteinase from 60 to 70-year-old human lenses.


Fractions		 Protein
(mg/ml)		 Enzyme
(e.u./ml)*		 Specific
activity**
1. α-crystallin-sodium deoxycholate-treatment 3.5 1.6 0.457
2. Agarose A 1.5 m chromatography 0.44 14.8 33.6
3. Non-denaturing gel electrophoresis 0.08 2.4 30
4. Size-exclusion HPLC 0.004 2 500

Proteinase activity was determined following detergent treatment of the α-crystallin fraction and size-exclusion HPLC using a TSK G-3000 PWXL column. An asterisk indicates that one enzyme unit (e.u.) is defined as the production of 0.1 nanomole of 7-amino 4-methyl coumarin per ml. The double asterisk denotes that specific activity is given in the measurement of e.u./mg of the protein.

Srivastava, Mol Vis 2008; 14:1872-1885. http://www.molvis.org/molvis/v14/a222

©2008 Molecular Vision http://www.molvis.org/molvis/

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