Figure 8. Proteinase activity in four
fractions (WS proteins, α-crystallin, βH-crystallin, and
membrane fractions) following treatment with sodium deoxycholate and
size-exclusion Agarose A1.5 m chromatography. WS proteins (A),
α-crystallin fraction (B), βH-crystallin fraction (C),
and membrane fraction (D). Each fraction was first isolated from
a homogenate of six pooled lenses of donors 30–40 years old by Agarose
A1.5 m chromatography. Next, each fraction was treated with the
detergent and fractionated by the size-exclusion chromatography, and
the column fractions were examined for their absorbance at 280 nm (red
line) and proteinase activity (green line) with BAPNA as a substrate.
Note that the proteinase activity eluted in the identical fractions
during chromatography from each of the four fractions.