Figure 5. Comparison of elution profiles at 280 nm during size-exclusion HPLC of purified proteinase from the α-crystallin fraction, recombinant βA3/A1-crystallin, and recombinant βB1-crystallin. The recombinant proteins were treated with 1% sodium deoxycholate (w/v) before their chromatography (TSK G3000 PW_XL). The proteinase activity was determined by the incubation of column fractions with BAPNA at 37 °C for 10 h.