**Figure 3. **SDS–PAGE analysis of various
fractions recovered during purification of α-crystallin-associated
fractions. Lane 1: α-Crystallin fraction was recovered from a WS
protein fraction of lenses that were 60- to 70-years-old. Lane 2:
Concentrated α-crystallin fraction applied to the Agarose A1.5 m
column. Lane 3: Proteinase fractions recovered following non-denaturing
gel electrophoresis of the proteinase fraction released after Agarose
A1.5 m gel chromatography. Lane 4: A proteinase-containing fraction
recovered following size-exclusion HPLC using TSK G-4000 PW_{XL}
column of the fraction recovered in lane 3.