Figure 3. SDS–PAGE analysis of various fractions recovered during purification of α-crystallin-associated fractions. Lane 1: α-Crystallin fraction was recovered from a WS protein fraction of lenses that were 60- to 70-years-old. Lane 2: Concentrated α-crystallin fraction applied to the Agarose A1.5 m column. Lane 3: Proteinase fractions recovered following non-denaturing gel electrophoresis of the proteinase fraction released after Agarose A1.5 m gel chromatography. Lane 4: A proteinase-containing fraction recovered following size-exclusion HPLC using TSK G-4000 PW_XL column of the fraction recovered in lane 3.