Figure 10. Time-dependent proteolysis of bovine recombinant γB-, γC-, and γD-crystallins by the human lens membrane proteinase. The incubation periods are shown at the bottom of the gel, and the reaction constituents are shown at the top of the gel. The proteolyzed fragments are identified with numbers, and only the fragments from γD-crystallin were used for partial NH₂-terminal sequence analysis.