Figure 1. SDS–PAGE analysis of the column fractions that contained proteinase activity after Agarose A1.5 m chromatography of the sodium deoxycholate-treated α-crystallin fraction. Note that two protein bands (identified as bands 1 and 2) with M_r of 20–25 kDa and the third band (identified as band 3) of 38 kDa were recovered.