Figure 2. Effect of fisetin on UVB-induced generation of reactive oxygen species in human lens epithelial cells. A: SRA01/04 cells were pretreated with 25 μg/ml fisetin for 1 h followed by exposure to 30 mJ/cm² UVB before incubation for 2 h and being loaded with DCFH-DA. Oxidant generation was measured by DCF flow cytometry as described in Methods. B: Representative fluorescent images of controls and UVB-irradiated cells were taken under fluorescence microscopy. Magnification, 100X.