Table 3 of Sharma,
Mol Vis 2008; 14:1856-1864. Table 3 of Sharma,
Mol Vis 2008; 14:1856-1864.
Table 3. The NHS-A mutations generated by PCR-based mutagenesis.
Mutation |
Protein length in amino acids (M wt. in kDa) |
Primer pairs for PCR (annealing temperature °C) | Restriction sites for cloning |
||
|---|---|---|---|---|---|
| PCR1 | PCR2 | PCR3 | |||
| 400delC R134fs) | 195 (21.5) | NHSF01 and NHSA400R (56) | NHSA400F and NHSA1350R (58) | NHSF01 and NHSA1350R (65) | EcoRI/EcoRV |
| C2701T (R901X) | 901 (99) | NHSA2220F and NHSA2701R (50) | NHSA2701F and NHSA3050R (52) | NHSA2220F and NHSA3050R (63) | HindIII/PmeI |
| 4071del299bp T1357fs) | 1367 (150) | NHSA4340F and NHSA4451R (56) | NHSA4451F and Tag2A810R (56) | NHSA4340F and Tag2A810R (62) | ScaI/SalI |