Molecular Vision: Sharma, Mol Vis 2008; 14:1856-1864. Table 1

Table 1. Primer sequences for the PCR amplification of NHS coding regions.

Exon	Amplimer	Forward Primer 5′→3′	Reverse Primer 5′→3′	Size (bp)	Mg²⁺ concentration (mM)	Annealing temperature (°C)
1	1A*	GCGCACCCCTAAATTTCT	CTGCCTGCACAGCCATTG	311	2	60
	1B*	GCTTGGAGGAGACCAGAAAGT	CCTGGAAGAGGCTGCAAG	494	2	62
	1C*	GCTGCTCATGCTGGACCTA	GGTGGGAAGGCGAGAGTAGT	349	1.5	60
2	2	TGCAGTAGTCTGGACTTCCACT	CCCATGGATTTCATTTCAGG	426	1.5	60
3	3	ACTCCCAAGGGGAAAAGAGA	CTGATGTTTCCTCAGCAGCA	257	1.5	60
1b	1b	TCTCTCTCTCTGTATGCACCAAA	TCATGCCCTGTTGACTCAGA	352	1.5	60
4	4	GGGCCTATTTCTGACCTCATT	AGCAGCACAGATTTTGAACA	377	1.5	60
5	5	TCTTTCCTTACTTCCCGTCAAA	CATCTGTACTAGGCGGAGGAA	282	1.5	60
6	6A	CTGCCAGCCCACAGATCTAC	GCTTGGAGCCTCACTGAAAT	501	1.5	60
	6B	TCCCCGGGAAGGTAATAGAG	TGAGGGGCTGTGTTTAGTGA	501	1.5	60
	6C	TGACCACCAGTCATCCAGTG	ACCACAGTCAAAGTGCATGG	505	1.5	60
	6D	CATCAGACAAAGCGGACACT	TGGTGGAATATCCGAAGCAT	503	1.5	60
	6E	AATGGAAAACGCCAATCTTC	AGAGGGTTGCTGTAGTGAGGA	502	1.5	60
	6F	CTTGGCATCTCCATCAAGTG	TGCGGCCTAATCTTACTTGG	498	1.5	60
	6G	CCTTGCAATTACACCAACGA	GAGGGTGCCTTCTGTACCTG	534	1.5	60
	6H	AAAACACGCCAACCAAAAAC	ATTCCAGGAAGTGCCATGAG	501	1.5	60
7	7	CAGTAGCGTGCTGGGTAACTT	GGGCAAAACCTTTGTTGTA	235	1.5	60
8	8A	TTTCATAAAAACGTGAACTGAGTGA	GCAAAGCTCTTCGAGGAAAA	489	1.5	60
	8B	CCAGAGGTCTCCTGGTCTCA	GTAAGGGTTTTGGCCTTTGC	511	1.5	60