Figure 4. Localization of FLAG-tagged wild type and mutant NHS-A proteins in MDCK cells. MDCK cells stably expressing FLAG-NHS-A, FLAG-NHS-AC2701T, and FLAG-NHS-A4071del299bp proteins and untransfected cells were immunolabeled with an anti-FLAG tag antibody, and labeling was detected by confocal microscopy. Wild type FLAG-NHS-A protein mainly localized to the cellular periphery whereas FLAG-NHS-AC2701T and FLAG-NHS-A4071del299bp mutant proteins localized in the cytoplasm. No non-specific immunoreactivity was observed in untransfected cells. Representative images from four independent experiments are shown. Images were taken with a 60X objective.