Figure 3. Localization of GFP-NHS-A400delC mutant in MDCK cells. Cells were transfected with GFP-NHS-A and GFP-NHS-A400delC fusion constructs and pEGFP-C1 control. Transiently expressed fusion proteins were visualized by confocal microscopy. GFP-NHS-A wild type protein primarily localized to the cellular periphery whereas GFP-NHS-A400delC mutant protein localized in the cytoplasm and nucleus. Apparent peripheral distribution of GFP is an experimental artifact seen only between some adjoining transfected cells. Images were taken with a 60X objective.