Figure 6. Rabbit lens α-crystallin phosphorylation. A shows the optical scan of a rabbit lens equatorial cryosection before MALDI matrix deposition. B shows the distribution of full-length αA-crystallin. C: Singly-phosphorylated full-length αA-crystallin (observed m/z=19953) shows higher abundance in the middle cortex. D: The dual color image shows the relationship between the spatial distributions of full-length αA-crystallin (red) and phosphorylated αA-crystallin (green). Both full-length αB-crystallin (E; residues 1–175, observed m/z=20146) and the singly-phosphorylated αB-crystallin (F; observed m/z=20215) show higher abundance in the middle cortex. G: The dual color image shows the relationship between the spatial distributions of full-length αB-crystallin (red) and phosphorylated αB-crystallin (green). Yellow coloration indicates colocalization. P=phosphorylated form of protein. Scale bar=2 mm.