Figure 5. Bovine lens α-crystallin phosphorylation. A shows the optical scan of a bovine lens equatorial cryosection before MALDI matrix deposition. B illustrates the distribution of full-length αA-crystallin. C: Singly-phosphorylated full-length αA-crystallin (observed m/z=19920) shows higher abundance in the middle cortex. D: The dual color image shows the relationship between the spatial distributions of full-length αA-crystallin (red) and phosphorylated αA-crystallin (green). E: The distribution of full-length αB-crystallin (residues 1–175, observed m/z=20084) showed higher abundance toward the edge of the lens and in the inner cortex. Singly- phosphorylated (F) and doubly- phosphorylated (G) αB-crystallin (observed m/z=20163 and 20248, respectively) are more abundant in the middle cortex. H: The dual color image shows the relationship between the spatial distributions of full-length αB-crystallin (red) and doubly-phosphorylated αB-crystallin (green). P/2P=phosphorylated forms of protein. Scale bar=2 mm.