Figure 2. Conserved Gln61 changed to Arg
was predicted to disturb an α-helix. p.Gln61>Arg substitution
changed one conserved Gln residue and was predicted to disturb an
α-helix. A: Secondary structure of HSF4b predicted using
PepTool Lite software (Biotools Inc., Edmonton, Alberta, Canada). B:
Multiple sequence alignments of HSF4 homologous sequences in different
species are shown. Mutations reported in this study are highlighted in
red; previously reported mutations are highlighted in black.