Figure 4 of Murugesan, Mol Vis 2008; 14:1835-1844.


Figure 4. Electron microscopic image of αBT162C in reduced and oxidized form. A drop of 1 mg/ml protein was negatively stained with 2% uranyl acetate and observed under the JOEL 1200EX electron microscope. A: Reduced form shows homogeneous population of oligomers of 10-15 nm. B: Oxidized αBT162C forms highly heterogeneous and larger aggregates. The EM analysis of reduced and oxidized αBT162C confirms the aggregation of αB-crystallin following oxidation and crosslinking.