Figure 2. Molar mass distribution and elution profile of mutant and wild-type αB-crystallin proteins under reducing conditions. Protein (0.1 mg each) in buffer was injected into a TSK5000 gel filtration column connected to a multi-angle light scattering instrument, and the data was analyzed as described under Methods. Blue, wild-type αB-crystallin; Green, αBI5C; Red, αBT162C. Molecular mass of αBI5C and αBT162C mutants was not significantly altered compared to that of wild-type αB-crystallin under reducing conditions.