Figure 2. Sphere formation from rabbit corneal fibroblasts. A: A representative day 7 sphere from rabbit corneal fibroblasts had a diameter of approximately 300 μm. B: Each primary sphere was positive for BrdU on day 7. C: The differentiated progeny from the primary sphere showed a typical fibroblast-like morphology. D: Secondary spheres were generated from dissociated primary spheres. E and F: Day 7 spheres were positive for keratocan. G and H: Progenies derived from the sphere are positive for keratocan. Scale bar=100 μm.