Figure 2 of Zhu, Mol Vis 2008; 14:1805-1814.

Figure 2. Representative two-dimensional gel images showing separation of proteins extracted from human corneal endothelial cells cultured from young and older donors. Extracted protein was pooled from five young (<30 years old; A,C) and five older donors (>50 years old; B,D). Equal amounts of protein were separated on either pH 3–10 IPG strips (A,B) or pH 4–7 IPG strips (C,D) followed by separation on 8%–16% polyacrylamide gels. Protein spots were stained with SyproRuby. Images were obtained using a ProEXPRESS Proteomic Imaging System.