Table 2 of Yuan, Mol Vis 2008; 14:1792-1798.

Table 2. Results of RNA quality control evaluation.

Experimental
group		 Scaling
factor		 Average
background		 PCall
%		 β-Actin
3′to 5′ ratio		 GAPDH
3′to 5′ ratio
Infected 1 2.5 64.5 56.9 2.6 2.7
Infected 2 2.5 54.1 54 2 2.1
Infected 3 2.4 55.8 52.4 1.8 1.9
Control 1 2.4 60.6 52.9 2.8 2.6
Control 2 2.4 54.9 53.7 3 2.6
Control 3 4.6 53.7 47.2 3.1 2.8

Triplicate samples of five-cornea pools were used from Candida albicans-infected or mock-infected control animals. PCall % is the percent of probe sets that are called “present” by Affymetrix’s algorithm. The 3′ to 5′ ratio is the 3′-end to 5′-end probe intensity ratio. The number of “present” probes for housekeeping probe sets (β-actin and GAPDH) was 3, and the number of spike-in probe sets (BioB, BioC, BioD, and CreX) was 2.

Yuan, Mol Vis 2008; 14:1792-1798. http://www.molvis.org/molvis/v14/a211

©2008 Molecular Vision http://www.molvis.org/molvis/

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