Figure 6. Quantitative real-time
polymerase chain reaction analyses of p53 mRNA expression in
human corneal endothelial cells at various ages. Total RNA was isolated
from HCECs of each group, and the first-strand cDNA were aynthesized
from equal amounts of total RNA. Quantitative real-time PCR was
performed to analyze p53 mRNA expression. A shows the
expression of p53 from the eight age groups normalized to GAPDH
mRNA. B demonstrates fold change in p53 expression
relative to the calibrator of the 20-year-old group. C is the
comparison of the average level of p53 mRNA expressed in HCECs
between the young (≤ 30 years) and old (≥ 50 years). Data are presented
as mean±standard deviation, and a p<0.05 is considered to be
statistically significant. Results indicate no significant increase
(p=0.430) in p53 mRNA expression in HCECs from the old donors.