Figure 2. Detection of
p16INK4a,
p21WAF1/CIP1,
p27KIP1, and
p53
mRNA. Electrophoretic gels show the presence of mRNA for the four
senescence-related genes. Lane 1 to Lane 5 shows HCECs of the 20-, 30-,
40-, 50-, and 60-year-old groups, respectively. Lane 6 is the negative
control. Lane M is Marker DL 2000. All bands migrate to their expected
product length as summarized in
Table 1.