Table 2 of Dibas, Mol Vis 2008; 14:1770-1783.

Table 2. Primer optimization.

Primer name Denaturation Annealing Extension Number of cycles
AQP4 95°C 30 s 60oC 30 s 72oC 60 s 45
UCH-L1 95oC 30 s 60oC 30 s 72oC 60 s 40
β-actin 95oC 60 s 60oC 60 s 72oC 120 s 40

All primers were optimized for annealing temperatures and cycles.

Dibas, Mol Vis 2008; 14:1770-1783. http://www.molvis.org/molvis/v14/a209

©2008 Molecular Vision http://www.molvis.org/molvis/

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