Figures 9 of Dibas, Mol Vis 2008; 14:1770-1783.


Figures 9. Elevation of IOP increased ubiquitination in retinal extracts and decreased ubiquitination in optic nerve extracts. Ubiquitination starts with monoubiquitination at multiple lysine residues then extending the ubiquitin chains by the sequential addition of multiple ubiquitin moieties and that is why when using the anti-ubiquitin antibodies, immunoreactive smear is detected that reflects the covalent attachment of multiple ubiquitin chains to individual protein molecule. Western blot with anti-ubiquitin antibodies revealed a characteristic ubiquitin-positive smear in each lane corresponding to the amount of ubiquitination on proteins, and elevation of IOP enhanced ubiquitination in retinal extracts (A) while decreased ubiquitination in optic nerve extracts (B). Abbreviations: C-R is control retina, and T-R is elevated IOP retina; C-ON is control optic nerve, and T-ON is elevated IOP optic nerve.