Figure 5 of Dibas, Mol Vis 2008; 14:1770-1783.


Figure 5. Procaspase-3 protein is upregulated following intravitreal ET-1 injection and elevation of intraocular pressure. Procaspase-3 (31 kDa) is normally cleaved to a 17 kDa fragment that is fully active. However, in both models, an upregulation of the procaspase-3 form was observed without detection of the 17 KDa fragment. A: Immunoreactive bands for procaspase-3 and α-tubulin 2 days after intravitreal injection of ET-1 showing a significant increase in procaspase-3 protein levels (300%). B: Quantitative measurement using western blot also showed that elevation of IOP increased procaspase-3 protein levels by ~ 180%. Isolated cytosolic proteins (50 μg) were loaded into each lane. Densitometric quantification is shown in figures C and D. Data are expressed as a ratio of the control value and each column represents mean±SEM. The asterisk denotes statistical significance of procaspase-3 in ET-injected retinas versus control (p<0.05) and the double asterisk denotes statistical significance of procaspase-3 in elevated IOP-retinas versus control (p<0.05), as determined by one-way ANOVA and Tukey multiple comparison test. Abbreviations: control eye (C), ET-injected eye (E), and elevated IOP (T).