Figure 4. Glial fibrillary acidic protein
(GFAP) protein is upregulated following intravitreal ET-1 injection and
elevation of intraocular pressure. Following retinal injuries with ET
injection or elevation of IOP, retinas were dissected and cytosolic
proteins were isolated. Fifty microgram protein was loaded into each
lane. GFAP, a cellular marker for retinal injury, was upregulated in
both injury models but with significant induction following ET
injection (380+100%) compared to elevation of IOP (133+10%).
Densitometric quantification is shown in C and D. Data
are expressed as a ratio of the control value and each column
represents mean±SEM. The asterisk denotes statistical significance of
GFAP in ET-injected retinas versus control (p<0.005) and the double
asterisk denotes statistical significance of GFAP in elevated
IOP-retinas versus control (p<0.005), as determined by one-way ANOVA
and Tukey multiple comparison test. Abbreviations: control eye (C),
ET-injected eye (E), and elevated IOP (T).