Figure 3 of Dibas, Mol Vis 2008; 14:1770-1783.


Figure 3. Intravitreal ET-1 injection and elevated intraocular pressure reduced AQP4 protein levels in rat retinas. Following retinal injuries with ET-injection or elevation of IOP, retinas were dissected and plasma membrane proteins were isolated. Thirty microgram protein was loaded into each lane. A: Immunoreactive bands for AQP4 and α-tubulin 2 days after intravitreal injection of ET-1 showing a significant reduction in AQP4 protein levels. Also, quantitative measurement using western blot showed that elevation of IOP decreased AQP4 protein levels by ~ 30% (C). Densitometric quantification is shown in B and D. Data are expressed as a ratio of the control value and each column represents mean±SEM. The asterisk denotes statistical significance of AQP4 in ET-injected retinas versus control (p<0.05) and the double asterisk denotes statistical significance of AQP4 in elevated IOP-retinas versus control (p<0.001) as determined by one-way ANOVA and Tukey multiple comparison test. Abbreviations: control eye (C), ET-injected eye (E), and elevated IOP (T).