Figure 2 of Dibas, Mol Vis 2008; 14:1770-1783.


Figure 2. A single intravitreous ET-1 injection and elevated intraocular pressure reduced AQP4 mRNA in rat retinas. Four microliters of HEPES-buffered ET-1 (2 nmol) or HEPES vehicle, was injected into the vitreous of the left eye and 2 days later, retinas were removed, total RNA was isolated and transcribed into cDNA. IOP was elevated using the Morrison Method [22]. mRNAs for AQP4 transcripts were significantly lower compared to controls following 2 days of ET-injection (~ 45%, A). Elevation of IOP also decreased AQP4 transcripts by ~ 33%, as determined by quantitative real-time PCR (B). Gene expression data of AQP4 is calculated after normalizing with β-actin. Data are expressed as ratio of control±SEM and the asterisk denotes a significant difference compared with control-retinas at p<0.05.