Figure 3. Serum antibody responses to AAV capsid in animals after different sets of sequential intraocular AAV2-CBA-GFP injection. Five animals for each group were used. Group 1 animals received two sequential intravitreal (IV) injections of the vector. Group 2 animals received two sequential subretinal (SR) injections. Group 3 received SR injection in first eye, followed by IV injection in the second eye. Group 4 animals received IV injection in the first eye, followed by SR injection in the second eye.