Table 3 of Fink, Mol
Vis 2008; 14:1737-1751. Table 3 of Fink, Mol
Vis 2008; 14:1737-1751.
Table 3. PCR-Primers for SNP-genotyping.
Gene |
Target |
SNP |
Primer |
Primer sequence (5′→3′) |
Annealing temperature (°C) |
Product size (bp) |
|---|---|---|---|---|---|---|
| TNNT1 | intron 10 | AM930546 g.273A>G | TNNT1_SNPex11_F | CAGAGTTGGGGATGGATATG | 58 | 597 |
| TNNT1_SNPex11_R2 | AGACCAGAGGGATGTGTTGG | |||||
| exon 11 | AM930555 c.359A>G | TNNT1_SNPex11_F | CAGAGTTGGGGATGGATATG | 58 | 597 | |
| TNNT1_SNPex11_R2 | AGACCAGAGGGATGTGTTGG | |||||
| exon 13 | AM930555 c.425A>C | TNNT_SNP_ex13_F2 | CTGACACCCCTCCTTCTCCT | 58 | 157 | |
| TNNTex8–13_R | CCCAGATGGACACACACC | |||||
| RDH13 | 5′UTR | AM930553 c.57A>C | RDH13_ex1_F_gen | GCGCTCTAGGTGCAGACTC | 60 | 381 |
| RDH13_ex1_R_gen | CCGGAAGCAACTAGACCAAA | |||||
| exon 1 | AM930553 c.103C>G | RDH13_ex1_F_gen | GCGCTCTAGGTGCAGACTC | 60 | 381 | |
| RDH13_ex1_R_gen | CCGGAAGCAACTAGACCAAA | |||||
| intron1 | AM930548 g.294C>T | RDH13_ex1_F_gen | GCGCTCTAGGTGCAGACTC | 60 | 381 | |
| RDH13_ex1_R_gen | CCGGAAGCAACTAGACCAAA | |||||
| exon 2 | AM930553 c.151C>T | RDH13_ex2_F_gen | CCTTGGGTTGTGGGATATTG | 60 | 338 | |
| RDH13_ex2_R_gen | CCAACCACCACCAGGTCTTA | |||||
| intron 2 | AM930549 g.8G>A | RDH13_ex3_F_gen | TTTTCTCGTCTGGCTCTTCC | 60 | 307 | |
| RDH13_ex3_R_gen | TCTTCACCCAAAGACGGAAC | |||||
| intron 3 | AM930550 g.137T>C | RDH13_ex4_F_gen | CCAGGTAGTTAACGCCAAGC | 58 | 372 | |
| RDH13_ex4_R_gen | TGCCTTTCTCTGGCTCACTT | |||||
| exon 5 | AM930553 c.703C>A | RDH13_SNPex5_F | GCCACTTCCTTTTGACGAAC | 60 | 355 | |
| RDH13_SNPex5_R | GTCGGGCCTAAGTGTGTCAT | |||||
| exon 7 | AM930554 c.491G>C | RDH13_SNPex7_F | GGTTCCTGCATCTGGAATTG | 60 | 381 | |
| RDH13_SNPex7_R | ACTTTCAGCCCAAAGCCTCT | |||||
| TFPT | exon 2 | AM930551 c.337A>T | TFPT_SNPex3_F | GGCTTGAGCTGTCCAGTGA | 60 | 387 |
| TFPT_SNPex3_R | TGCGATTTAGTTCCCTCTGG | |||||
| exon 2 | AM930551 c.379G>T | TFPT_SNPex3_F | GGCTTGAGCTGTCCAGTGA | 60 | 387 | |
| TFPT_SNPex3_R | TGCGATTTAGTTCCCTCTGG | |||||
| exon 5 | AM930552 c.176G>A | TFPT_exon6_SNP_genF | CTCTCCCGTCTGCCAGGAT | 62 | 297 | |
| TFPT_exon6_SNP_genR | CTTTCCCTGCTTCCCCTGT |