Table 2 of Fink, Mol
Vis 2008; 14:1737-1751. Table 2 of Fink, Mol
Vis 2008; 14:1737-1751.
Table 2. cDNA PCR primers.
Gene |
Target |
Primer |
Primer location |
Primer sequence (5′-3′) |
Annealing Temperature (°C) |
Product Size (bp) |
|---|---|---|---|---|---|---|
| TNNT1 | cDNA | TNNT1ex1–9_F | Exon 1 | GCCGAAGAGCAAGAATATGA | 58 °C | 492 |
| TNNT1ex1–9_R | Exon 9 | GACCAGATAACCCCCAAAAT | ||||
| TNNT1ex8–13_F | Exon 8 | GCTTCAGAACCGAGAAGGA | 58 °C | 533 | ||
| TNNTex8–13_R | Exon 13 | CCCAGATGGACACACACC | ||||
| TNNT_ex4_F_cDNA | Exon 1/2 | AGAGGAGCAGCCCTGAAGA | 60 °C | 172 | ||
| TNNT_ex4_R_cDNA | Exon 6 | ATGTCATCGAAGTCCACACG | ||||
| RDH13 | cDNA | RDH13_cF1 | Exon 1 | GCGCTCTAGGTGCAGACTCCG | 60 °C | 714 |
| RDH13_cR1 | Exon 5 | CTCAGCTCCTTGGTGGAGAC | ||||
| RDH13_cF2 | Exon 3 | GAAGTCCATCCGAGAGTTCG | 60 °C | 789 | ||
| RDH13_cR2 | Exon 7 | GGCATGACAGCTAGGTTTGG | ||||
| TFPT | cDNA | TFPT_ex1_F | Exon 1 | GAGCCCGATAAACAGACTCG | 60 °C | 655 |
| TFPTex1–2_R | Exon 3 | GCTGGAGTCTCCGAGTTATTC | ||||
| TFPTex1–2_F | Exon 2 | GTGGGCTTCGAGGAGTTC | 60 °C | 689 | ||
| TFPT_ex6_R | Exon 6 | TAGGGCAGCAGTTTGTCTGG |